慢病毒载体基因
北京义翘神州在克隆技术方面拥有丰富的经验。凭借独特的载体设计系统和最大的表达型ORF cDNA克隆的优势，ORF可以在很短的时间内穿梭到慢病毒载体中。慢病毒载体基因的优势
多种属| • 18000+ 人慢病毒载体基因 | • 10000+ 小鼠慢病毒载体基因 | • 1000+ 病毒慢病毒载体基因 |
| • 5000+ 大鼠慢病毒载体基因 | • 2000+ 狗慢病毒载体基因 | • 1000+ 其他种属慢病毒载体基因 |
| • 2000+ 食蟹猴/恒河猴慢病毒载体基因 |
全长序列验证
第三代慢病毒载体
• 包含特定的元素来提高转基因表达,病毒效价,生物安全
部分慢病毒载体基因列表
更多慢病毒载体基因产品| Molecule | Catalog | Tag | Availbility |
| PD1/PDCD1/CD279 | HG10377-UTLN | untagged | in stock |
| HG10377-CFLN | C-Flag | in stock | |
| HG10377-ACGLN | C-GFPSpark | in stock | |
| PD-L1/B7-H1/CD274 | HG10084-UTLN | untagged | in stock |
| HG10084-CFLN | C-Flag | in stock | |
| HG10084-ACGLN | C-GFPSpark | in stock | |
| PD-L2/B7-DC/CD273 | HG10292-UTLN | untagged | in stock |
| HG10292-CFLN | C-Flag | in stock | |
| HG10292-ACGLN | C-GFPSpark | in stock | |
| CD80/B7-1 | HG10698-UTLN | untagged | in stock |
| HG10698-CFLN | C-Flag | in stock | |
| HG10698-ACGLN | C-GFPSpark | in stock | |
| CD86/B7-2 | HG10699-UTLN | untagged | in stock |
| HG10699-CFLN | C-Flag | in stock | |
| HG10699-ACGLN | C-GFPSpark | in stock | |
| CD28/TP44 | HG11524-UTLN | untagged | in stock |
| HG11524-CFLN | C-Flag | in stock | |
| HG11524-ACGLN | C-GFPSpark | in stock | |
| CTLA-4/CD152 | HG11159-UTLN | untagged | in stock |
| HG11159-CFLN | C-Flag | in stock | |
| HG11159-ACGLN | C-GFPSpark | in stock | |
| VEGF/VEGFA | HG11066-UTLN | untagged | in stock |
| HG11066-CFLN | C-Flag | in stock | |
| HG11066-ACGLN | C-GFPSpark | in stock | |
| HER2/ERBB2 | HG10004-UTLN | untagged | in stock |
| HG10004-CFLN | C-Flag | in stock | |
| HG10004-ACGLN | C-GFPSpark | in stock | |
| ERBB3/HER3 | HG10201-UTLN | untagged | in stock |
| HG10201-CFLN | C-Flag | in stock | |
| HG10201-ACGLN | C-GFPSpark | in stock | |
| VEGFR2/KDR/Flk-1/CD309 | HG10012-UTLN | untagged | in stock |
| HG10012-CFLN | C-Flag | in stock | |
| HG10012-ACGLN | C-GFPSpark | in stock | |
| TNF-alpha/TNFA/TNFSF2 | HG10602-UTLN | untagged | in stock |
| HG10602-CFLN | C-Flag | in stock | |
| HG10602-ACGLN | C-GFPSpark | in stock | |
| TP53/p53 | HG10182-UTLN | untagged | in stock |
| HG10182-CFLN | C-Flag | in stock | |
| HG10182-ACGLN | C-GFPSpark | in stock | |
| ANG1/ANGPT1 | HG13667-UTLN | untagged | in stock |
| HG13667-CFLN | C-Flag | in stock | |
| HG13667-ACGLN | C-GFPSpark | in stock |
慢病毒对照载体
使用对照载体与慢病毒包装系统一起共转染以制备重组慢病毒.
| Description | Catalog | Size | Availbility |
| GFP Lentivirus Control Plasmids | LVCV-01 | 10 ug | in stock |
| OFP Lentivirus Control Plasmids | LVCV-02 | 10 ug | in stock |
GFP慢病毒生产和转导
- GFP慢病毒对照质粒和包装载体共转染293T细胞。
- 转染36-72小时后收获慢病毒上清液。
- 用慢病毒上清液感染293FT细胞以测定慢病毒滴度。
- 收获细胞并进行qRT-PCR和流式细胞仪检测慢病毒滴度.
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