商品介绍
JAK2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: General Information
Gene
Species
Human
NCBI Ref Seq
NM_004972.3
RefSeq ORF Size
3438 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 489C/T,2490G/A not causing the amino acid variation.
Description
Full length Clone DNA of Human Janus kinase 2 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI(Three restriction sites) + NotI(6kb+2.16kb+1.23kb+0.06kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
JAK2 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
JAK2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Validated Images
JAK2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Synonyms
JTK10 cDNA ORF Clone, Human; THCYT3 cDNA ORF Clone, Human
JAK2 Background Information
Full Name
Janus kinase 2
Research Areas
- Cancer Drug Targets
Related Pathways
EGFR Signaling Pathway
Jak-Stat Signaling Pathway
Common G-Chain signaling pathway
IL12 signaling pathway
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- Human JAK2 qPCR Primer PairHP101103
- Accumulation of JAK activation loop phosphorylation is linked to type I JAK inhibitor withdrawal syndrome in myelofibrosisAuthorTvorogov, D;Thomas, D;Liau, NPD;Dottore, M;Barry, EF;Lathi, M;Kan, WL;Hercus, TR;Stomski, F;Hughes, TP;Tergaonkar, V;Parker, MW;Ross, DM;Majeti, R;Babon, JJ;Lopez, AF;Year2018JournalSci AdvApplicationexpressionPubMed ID: 30498775FoldedExpand
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