商品介绍
LDLR/LDL Receptor Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
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Activity
Measure by its ability to bind with human PCSK9 in a functional ELISA.1. Immobilized human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.61 μg/ml.2. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain of human LDLR (NP_000518.1) precursor (Met 1-Arg 788) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_000518.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 22
Molecule Mass
The secreted recombinant human LDLR comprises 777 amino acids with a predicted molecular mass of 86 kDa. As a result of different glycosylation, it migarates with the apparent molecular mass of 110-140 kDa in SDS-PAGE under reducing conditions corresponding to the mature and immature form.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
LDLR/LDL Receptor Protein, Human, Recombinant (His Tag): Images
Measure by its ability to bind with human PCSK9 in a functional ELISA. Immobilized human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.61 μg/ml.
Measure by its ability to bind with human PCSK9 in a functional ELISA. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 μg/ml.
LDLR/LDL Receptor Protein, Human, Recombinant (His Tag): Synonyms
FH Protein, Human; FHC Protein, Human; LDL R Protein, Human; LDL Receptor Protein, Human; LDLCQ2 Protein, Human
LDLR/LDL Receptor Background Information
LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.
Full Name
low density lipoprotein receptor
References
- Yamamoto T, et al. (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell. 39(1): 27-38.
- Mao B, et al. (2001) LDL-receptor-related protein 6 is a receptor for Dickkopf proteins. Nature. 411(6835): 321-5.
- Pinson KI, et al. (2000) An LDL-receptor-related protein mediates Wnt signalling in mice. Nature. 407(6803): 535-8.
Human LDLR/LDL Receptor Proteins
- LDLR/LDL Receptor Protein, Human, Recombinant (193D/A, ECD, His Tag)10231-H08H1
- LDLR/LDL Receptor Protein, Human, Recombinant (mFc Tag)10231-H05H
- LDLR/LDL Receptor Protein, Human, Recombinant (His Tag), Biotinylated10231-H08H-B
Mouse LDLR/LDL Receptor Proteins
- LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag)50305-M08H
- LDLR/LDL Receptor Protein, Mouse, Recombinant (His Tag), Biotinylated50305-M08H-B
- Optimization and in Vivo Validation of Peptide Vectors Targeting the LDL ReceptorAuthorJacquot, G;Lécorché, P;Malcor, JD;Laurencin, M;Smirnova, M;Varini, K;Malicet, C;Gassiot, F;Abouzid, K;Faucon, A;David, M;Gaudin, N;Masse, M;Ferracci, G;Dive, V;Cisternino, S;Khrestchatisky, M;Year2016JournalMol. Pharm.ApplicationSPRPubMed ID: 27656777FoldedExpand
- Identification and characterization of highly versatile peptide-vectors that bind non-competitively to the low-density lipoprotein receptor for in vivo targeting and delivery of small molecules and protein cargosAuthorDavid, M;Lécorché, P;Masse, M;Faucon, A;Abouzid, K;Gaudin, N;Varini, K;Gassiot, F;Ferracci, G;Jacquot, G;Vlieghe, P;Khrestchatisky, M;Year2018JournalPLoS ONEApplicationSPRPubMed ID: 29485998FoldedExpand
- The Immunoglobulin Superfamily Receptome Defines Cancer-Relevant Networks Associated with Clinical OutcomeAuthorVerschueren, E;Husain, B;Yuen, K;Sun, Y;Paduchuri, S;Senbabaoglu, Y;Lehoux, I;Arena, TA;Wilson, B;Lianoglou, S;Bakalarski, C;Franke, Y;Chan, P;Wong, AW;Gonzalez, LC;Mariathasan, S;Turley, SJ;Lill, JR;Martinez-Martin, N;Year2020JournalCellApplicationSPRPubMed ID: 32589946FoldedExpand
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