Sino Biological/B7-H3 ELISA Kit, Human/1/KIT11188,Sino Biological,,Sino Biological,sinobiological,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Sino Biological/B7-H3 ELISA Kit, Human/1/KIT11188

产品编号：KIT11188

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Sino Biological

公司：SinoBiological

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B7-H3 ELISA Kit, Human: General Information

Product name

B7-H3 ELISA Kit, Human

Assay type

Solid Phase Sandwich ELISA (quantitative)

Conjugate

HRP

Sample type

Serum,Urine

Sensitivity

16.20 pg/mL

Assay range

23.44-1500 pg/mL

Specificity

Recognizes both recombinant and natural Human B7-H3

Interference

Preparations of human B7-1, B7-2 and PD-L2at 50 ng/mL in a mid-range recombinant human B7H3 control were assayed for interference. No significant interference was observed.

Precision

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
N	20	20	20	20	20	20
Mean(pg/mL)	264.28	490.13	932.43	272.45	547.73	1,022.38
SD	6.32	12.00	25.22	13.81	23.71	32.98
CV(%)	2.4%	2.4%	2.7%	5.1%	4.3%	3.2%

	Intra-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	264.28	490.13	932.43
SD	6.32	12.00	25.22
CV(%)	2.4%	2.4%	2.7%
	Inter-assay Precision
Sample	1	2	3
N	20	20	20
Mean(pg/mL)	272.45	547.73	1,022.38
SD	13.81	23.71	32.98
CV(%)	5.1%	4.3%	3.2%

Recovery

The recovery of Human CD276 spiked to different levels throughout the range of the assay in related matrices was evaluated.

Sample	Average % Recovery	Range
serum (n=3)	86	80 -90%
urine (n=3)	107	106 -109%

Sample
serum (n=3)
Average % Recovery
86
Range
80 -90%
Sample
urine (n=3)
Average % Recovery
107
Range
106 -109%

Linearity

Serum

	Recovery of detected
1:2	109%
1:4	130%
1:8	123%
1:16	107%

Urine

	Recovery of detected
1:2	92%
1:4	108%
1:8	118%
1:16	99%

Materials provided

1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution

Product overview

This B7-H3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human B7-H3 protein in Serum,Urine . It contains recombinant Human B7-H3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Shipping

This ELISA Kit is shipped at ambient temperature.

Storage

Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA

B7-H3 ELISA Kit, Human: Images

Human B7-H3/CD276 ELISA Kit Standard Curve 14508

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Human B7-H3/CD276 ELISA Kit Specificity 14524

This assay recognizes both recombinant and natural Human B7H3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

B7-H3 ELISA Kit, Human: Synonyms

4Ig-B7-H3 ELISA Kit, Human; B7-H3 ELISA Kit, Human; B7H3 ELISA Kit, Human; B7RP-2 ELISA Kit, Human

B7-H3 Background Information

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Cancer Immunotherapy
Co-inhibitory Immune Checkpoint Targets
Immune Checkpoint
Immune Checkpoint Detection: Antibodies
Immune Checkpoint Detection: ELISA Antibodies
Immune Checkpoint Detection: FCM Antibodies
Immune Checkpoint Detection: ICC Antibodies
Immune Checkpoint Detection: IP Antibodies
Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets
Immunotherapy
Targeted Therapy

Full Name

CD276 molecule

Research Areas

Cancer Drug Targets

References

Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.

B7-H3 Matched ELISA Antibody Pair Set,Human
Assay Range(pg/ml):9.38-600
SEK11188

MicroRNA-199a Inhibits Cell Proliferation, Migration, and Invasion and Activates AKT/mTOR Signaling Pathway by Targeting B7-H3 in Cervical Cancer
Author
Yang, X;Feng, KX;Li, H;Wang, L;Xia, H;
Year
2020
Journal
Technol. Cancer Res. Treat.
Application
ELISA
PubMed ID: 32856542FoldedExpand

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