商品介绍
CEACAM6 Matched ELISA Antibody Pair Set,Human General Information
Product name
CEACAM6 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human CEACAM6
Assay range
15.6-1000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-CEACAM6 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in PBS before coating.2. Detection Antibody 0.4 mg/mL of biotinylated rabbit anti-CEACAM6 polyclonal antibody. Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 88 ng of recombinant CEACAM6. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.4. Streptavidin-HRP 50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000 Dilution in detection antibody dilution buffer before use.
Product overview
This CEACAM6 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CEACAM6 . It contains Human CEACAM6 capture antibody, Human CEACAM6 detector antibody (Biotin)Streptavidin-HRPand a highly purifiedHEK293-expressedrecombinant Human CEACAM6 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Detection Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.Streptavidin-HRP: Store at 4℃ and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
CEACAM6 Matched ELISA Antibody Pair Set,Human Images
CEACAM6 Matched ELISA Antibody Pair Set,Human: Synonyms
CD66c Matched ELISA Antibody Pair Set, Human; CEA Matched ELISA Antibody Pair Set, Human; CEAL Matched ELISA Antibody Pair Set, Human; NCA Matched ELISA Antibody Pair Set, Human
CEACAM6 Background Information
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), also known as nonspecific crossreacting antigen (NCA) and CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily. It s a glycosylphosphatidylinositol-linked immunoglobulin superfamily member that is overexpressed in a variety of human cancers, including colon, breast and lung and is associated with tumourigenesis, tumour cell adhesion, invasion and metastasis. CEACAM6 is a unique mediator of migration and invasion of drug resistant oestrogen-deprived breast cancer cells, and this protein could be an important biomarker of metastasis. CEACAM6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
Full Name
carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
References
- Duxbury MS, et al. (2004) Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness. Oncogene. 23(34): 5834-42.
- Lewis-Wambi JS, et al. (2008) Overexpression of CEACAM6 promotes migration and invasion of oestrogen-deprived breast cancer cells. Eur J Cancer. 44(12): 1770-9.
- Influences of enteral nutrition upon CEACAM6 expression by intestinal epithelial cellsAuthorKeenan, JI;Hooper, EM;Tyrer, PC;Day, AS;Year2013JournalInnate ImmunApplicationELISAPubMed ID: 24326999FoldedExpand
- The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in CholangiocarcinomaAuthorRose, JB;Correa-Gallego, C;Li, Y;Nelson, J;Alseidi, A;Helton, WS;Allen, PJ;D"Angelica, MI;DeMatteo, RP;Fong, Y;Kingham, TP;Kowdley, KV;Jarnagin, WR;Rocha, FG;Year2016JournalPLoS ONEApplicationELISAPubMed ID: 26974538FoldedExpand
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