商品介绍
SLITL2/VASN ELISA Kit, Human: General Information
Product name
SLITL2/VASN ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
3.83 pg/mL
Assay range
39.06-2500 pg/mL
Specificity
Recognizes both recombinant and natural Human SLITL2/VASN
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 386.00 | 757.00 | 1,606.00 |
| SD | 35.44 | 26.57 | 64.89 |
| CV(%) | 9.2% | 3.5% | 4.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 456.00 | 830.00 | 1,633.00 |
| SD | 51.05 | 52.30 | 145.49 |
| CV(%) | 11.2% | 6.3% | 8.9% |
Recovery
The recovery of Human VASN spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 113 |
| Range |
| 106-119% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 99% |
| 1:4 | 100% |
| 1:8 | 100% |
| 1:16 | 101% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This SLITL2/VASN ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human SLITL2/VASN protein in Serum . It contains recombinant Human SLITL2/VASN, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
SLITL2/VASN ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human VASN. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
SLITL2/VASN ELISA Kit, Human: Synonyms
SLITL2 ELISA Kit, Human
SLITL2/VASN Background Information
Vasorin is a typical type I membrane protein. It contains 1 EGF-like domain, 1 fibronectin type-III domain, 10 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. Vasorin is predominantly expressed in vascular smooth muscle cells, and that its expression is developmentally regulated. vasorin It directly binds to transforming growth factor (TGF)-β and attenuates TGF-β signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall.
Full Name
vasorin
References
- Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.
- Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
- Lee KA, et al. (2011) Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels. J Biol Chem. 286(48):41530-8.
- Identification of Functional mimotopes of human Vasorin Ectodomain by BiopanningAuthorLi, D;Zhang, T;Yang, X;Geng, J;Li, S;Ding, H;Li, H;Huang, A;Wang, C;Sun, L;Bai, C;Zhang, H;Li, J;Dong, J;Shao, N;Year2018JournalInt. J. Biol. Sci.ApplicationELISAPubMed ID: 29725267FoldedExpand
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