商品介绍
REG4 Matched ELISA Antibody Pair Set,Human General Information
Product name
REG4 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human REG4
Assay range
3.91-250 pg/mL
Materials provided
1. Capture Antibody 0.3 mg/mL of mouse anti-REG4 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 11186-MM05)2. Detection Antibody 0.5 mg/mL mouse anti-REG4 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11186-MM01)3. Standard Each vial contains 14 ng of recombinant REG4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.25 ng/mL is recommended.
Product overview
This REG4 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human REG4 . It contains Human REG4 capture antibody, Human REG4 detector antibody (HRP)and a highly purifiedHEK293-expressedrecombinant Human REG4 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
REG4 Matched ELISA Antibody Pair Set,Human Images
REG4 Matched ELISA Antibody Pair Set,Human: Synonyms
GISP Matched ELISA Antibody Pair Set, Human; REG-IV Matched ELISA Antibody Pair Set, Human; RELP Matched ELISA Antibody Pair Set, Human
REG4 Background Information
Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.
Full Name
regenerating islet-derived family, member 4
References
- Li FY, et al. (2010) RegIV expression showing specificity to gastrointestinal tract and its potential role in diagnosing digestive tract neuroendocrine tumor. J Zhejiang Univ Sci B. 11(4):258-66.
- Rafa L, et al. (2010) REG4 acts as a mitogenic, motility and pro-invasive factor for colon cancer cells. Int J Oncol. 36(3): 689-98.
- Hu G, et al. (2010) Purification of a bioactive recombinant human Reg IV expressed in Escherichia coli. Protein Expr Purif. 69(2): 186-90.
- REG4 Is Highly Expressed in Mucinous Ovarian Cancer: A Potential Novel Serum BiomarkerAuthorLehtinen, L;Vesterkvist, P;Roering, P;Korpela, T;Hattara, L;Kaipio, K;Mpindi, JP;Hynninen, J;Auranen, A;Davidson, B;Haglund, C;Iljin, K;Grenman, S;Siitari, H;Carpen, O;Year2016JournalPLoS ONEApplicationELISA (serum sample)PubMed ID: 26981633FoldedExpand
- Prognostic and diagnostic value of REG4 serum and tissue expression in pancreatic ductal adenocarcinomaAuthorSaukkonen, K;Hagström, J;Mustonen, H;Lehtinen, L;Carpen, O;Andersson, LC;Seppänen, H;Haglund, C;Year2018JournalTumour Biol.ApplicationELISA (serum)PubMed ID: 29542402FoldedExpand
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