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Sino Biological/Dopamine D2 Receptor/DRD2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag/1/HG12111-CF
产品编号：HG12111-CF
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Sino Biological/Dopamine D2 Receptor/DRD2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag/1/HG12111-CF
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Dopamine D2 Receptor/DRD2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: General Information

Gene
Species
Human
NCBI Ref Seq
NM_000795.3
RefSeq ORF Size
1371 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human dopamine receptor D2 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + XbaI(6kb+1.37kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Dopamine D2 Receptor/DRD2 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Dopamine D2 Receptor/DRD2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Validated Images

other Green fluorescent protein / GFP Gene Plasmid Map 5609
Human Dopamine D2 Receptor/DRD2 Gene Expression validated Image 16132
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Dopamine D2 Receptor/DRD2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Synonyms

D2DR cDNA ORF Clone, Human; D2R cDNA ORF Clone, Human

Dopamine D2 Receptor/DRD2 Background Information

Full Name
dopamine receptor D2

Dopamine D2 Receptor/DRD2 cDNA ORF Clone, Human,Related Products

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  • Human Dopamine D2 Receptor/DRD2 qPCR Primer PairHP101638
  • Aripiprazole and haloperidol protect neurite lesions via reducing excessive D2R-DISC1 complex formation
    Author
    Zheng, P;Hu, M;Xie, Y;Yu, Y;Jaaro-Peled, H;Huang, XF;
    Year
    2018
    Journal
    Prog. Neuropsychopharmacol. Biol. Psychiatry
    Application
    expression
    PubMed ID: 30597182FoldedExpand

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