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Sino Biological/SUPT5H cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag/1/HG18941-NF
产品编号：HG18941-NF
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Sino Biological/SUPT5H cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag/1/HG18941-NF
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SUPT5H cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: General Information

Gene
Species
Human
NCBI Ref Seq
NM_001111020.2
RefSeq ORF Size
3303 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 201C/T not causing the amino acid variation.
Description
Full length Clone DNA of Human SPT5 homolog, DSIF elongation factor subunit with N terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-FLAG
Restriction Sites
KpnI + NotI(6kb+3.3kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

SUPT5H cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

More Information About SUPT5H

Entrez Gene ID
6829

SUPT5H cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: Validated Images

Human GAPDH Gene Plasmid Map 5659

SUPT5H cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: Synonyms

SPT5 cDNA ORF Clone, Human; SPT5H cDNA ORF Clone, Human; SUPT5H cDNA ORF Clone, Human; Tat-CT1 cDNA ORF Clone, Human

SUPT5H Background Information

SUPT5H, a suppressor of Ty homolog-5 (SPT5), was identified as a novel tumor-specific hTERT promoter-binding protein and activator in colon cancer cells. The N terminus of SUPT5H is highly acidic, while the C terminus contains 7 mitogen-activated protein (MAP) kinase consensus sequences. SUPT5H is an evolutionarily conserved component of the DRB sensitivity-inducing factor complex that regulates RNA Polymerase II dependent mRNA processing and transcription elongation. SUPT5H may potentially be used as a novel tumor biomarker and/or cancer therapeutic target.
References
  • Vos SM, et al. (2018) Structure of activated transcription complex pol ii-dsif-paf-spt6. Nature 560 (7720): 607-612.
  • Schuelein R, et al. (2018) Targeting of rna polymerase ii by a nuclear legionella pneumophila dot/icm effector snpl. Cell Microbiol 20 (9): e12852.
  • Chen R, et al. (2015) Suppressor of ty homolog-5, a novel tumor-specific human telomerase reverse transcriptase promoter-binding protein and activator in colon cancer cells. Oncotarget 6 (32): 32841-32855.

SUPT5H cDNA ORF Clone, Human,Related Products

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  • SUPT5H cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tagHG18941-CF
  • SUPT5H Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tagHG18941-ACGLN
  • SUPT5H cDNA ORF Clone, Human, C-GFPSpark® tagHG18941-ACG
  • SUPT5H cDNA ORF Clone, Human, C-OFPSpark® tagHG18941-ACR
  • SUPT5H cDNA ORF Clone, Human, N-His tagHG18941-NH
  • SUPT5H cDNA ORF Clone, Human, N-OFPSpark® tagHG18941-ANR
  • SUPT5H cDNA ORF Clone, Human, N-HA tagHG18941-NY
  • SUPT5H cDNA ORF Clone in Cloning Vector, HumanHG18941-U
  • SUPT5H cDNA ORF Clone, Human, C-His tagHG18941-CH
  • SUPT5H cDNA ORF Clone, Human, C-Myc tagHG18941-CM
  • SUPT5H cDNA ORF Clone, Human, N-Myc tagHG18941-NM
  • SUPT5H cDNA ORF Clone, Human, untaggedHG18941-UT
  • Distinct Cdk9-phosphatase switches act at the beginning and end of elongation by RNA polymerase II
    Author
    Parua, P;Kalan, S;Benjamin, B;Sansó, M;Fisher, R;
    Year
    2020
    Journal
    bioRxiv
    Application
    template
    FoldedExpand

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中国生物科技股份有限公司是全球最大的cDNA、重组蛋白和抗体生产商之一。在流感病毒和其他致病性病毒的研究和开发方面，华生物正在迅速领先世界。中生生物所有试剂产品均由公司生产和质量控制，质量保证优于世界其他试剂供应商。它提供了不同菌株的各种抗体、蛋白质和cDNA克隆。临床前动物试验中使用了100多种抗体，抗体产品的检测灵敏度是国际主流试剂供应商的2-10倍

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