Sino Biological/Anti-HA tag Antibody, Mouse Monoclonal/1/100028-MM10,Sino Biological,,Sino Biological,sinobiological,+,,Array.Array蚂蚁淘厂家直采.

产品名称：Sino Biological/Anti-HA tag Antibody, Mouse Monoclonal/1/100028-MM10

产品编号：100028-MM10

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>聚合物>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Sino Biological

公司：SinoBiological

公司分类：

商品介绍

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Anti-HA tag Antibody, Mouse Monoclonal General Information

Product name

Anti-HA tag Antibody, Mouse Monoclonal

Validated applications

WB,ELISA,FCM,ICC/IF,IP

Specificity

HA tag

Immunogen

A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).

Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Source

Monoclonal Mouse IgG1 Clone #10

Purification

Protein A

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Concentration

0.5 μl/Test

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HA tag Antibody, Mouse Monoclonal Validated Applications

Application	Dilution
WB	1/2000-1/5000
ELISA	1/2000-1/5000
ICC-IF	1/200-1/500
IP	2-8 μL/mg of lysate

Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HA tag Antibody, Mouse Monoclonal Images

HA Enzyme linked immunosorbent assay (ELISA) 257

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HA-tagged protein (amino-terminus HA tag or carboxy-terminus HA tag).

Profile of anti-HA Tag reactivity on 293 transfected cells analyzed by flow cytometry. 293 cells transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D) were fixed and permeabilized according to the manufacturer"s manual (Cat. No. 554714,BD Pharmingen) and subsequently stained with Purified Mouse Anti-HA Tag antibody (Cat No. 100028-MM10) Or positive control antibody for 30 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 30 min on ice. Cells were washed twice and analyzed by flow cytometry.

Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (L) or mock-transfected (R). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SBI Mouse anti-HA-tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green).

Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).

Anti-HA Tag was immunoprecipitated using:

Lane A: HA-ARG1-myc transfected 293 cell lysate (0.5mg)

Lane B: myc-ARG1-HA transfected 293 cell lysate (0.5mg)

2 μL anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-HA Tag rabbit monoclonal antibody, at 1:100 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-HA Tag mouse monoclonal antibody at 1:1000 dilution

Lane A: HA-GST (Recombinant protein)(30ng)

Lane B: GST-HA (Recombinant protein)(10ng)

Lane C: HA-ARG1-myc transfected 293 cell lysate (2ug)

Lane D: HA-mFABP4-myc transfected 293 cell lysate (2ug)

Lane E: myc-mFABP4-HA transfected 293 cell lysate (0.5ug)

Lane F: myc-ARG1-HA transfected 293 cell lysate (2ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

HA tag Background Information

The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-tag for the detection of fusion proteins. Antibodies to HA-tag are the key of the most important tools in the detection and often used in purification of HA tag-related proteins, study of the protein interaction and detection of target protein expression.

References

Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

Anti-HA tag Antibody, Mouse Monoclonal
【Mouse】【Monoclonal】【IP】
100028-MM15
Anti-HA tag Antibody (HRP)
【Mouse】【Monoclonal】【ELISA】
100028-MM10-H

Infection-derived lipids elicit an immune deficiency circuit in arthropods
Author
Shaw, DK;Wang, X;Brown, LJ;Chávez, AS;Reif, KE;Smith, AA;Scott, AJ;McClure, EE;Boradia, VM;Hammond, HL;Sundberg, EJ;Snyder, GA;Liu, L;DePonte, K;Villar, M;Ueti, MW;de la Fuente, J;Ernst, RK;Pal, U;Fikrig, E;Pedra, JH;
Year
2017
Journal
Nat Commun
Application
WB/IP
PubMed ID: 28195158FoldedExpand

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中国生物科技股份有限公司是全球最大的cDNA、重组蛋白和抗体生产商之一。在流感病毒和其他致病性病毒的研究和开发方面，华生物正在迅速领先世界。中生生物所有试剂产品均由公司生产和质量控制，质量保证优于世界其他试剂供应商。它提供了不同菌株的各种抗体、蛋白质和cDNA克隆。临床前动物试验中使用了100多种抗体，抗体产品的检测灵敏度是国际主流试剂供应商的2-10倍

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