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Sino Biological/Anti-His tag Antibody, Mouse Monoclonal/1/105327-MM02T
产品编号：105327-MM02T
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Sino Biological/Anti-His tag Antibody, Mouse Monoclonal/1/105327-MM02T
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Anti-His tag Antibody, Mouse Monoclonal General Information

Product name
Anti-His tag Antibody, Mouse Monoclonal
Validated applications
WB
Specificity
His tag
Immunogen
A synthetic peptide corresponding to 6×His
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-His tag Antibody, Mouse Monoclonal Validated Applications

ApplicationDilution
WB1/1000-1/10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-His tag Antibody, Mouse Monoclonal Images

His tag Western blot (WB) 20945

Detection Limit

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: His-Human PA2G4(10ng)

Lane B: His-Human PA2G4(5ng)

Lane C: His-Human PA2G4(2ng)

Lane D: His-Human PA2G4(1ng)

Lane E: His-Human PA2G4(0.5ng)

Lane F: His-Human PA2G4(0.2ng)

Lane G: Human B7-2-His(10ng)

Lane H: Human B7-2-His(5ng)

Lane I: Human B7-2-His(2ng)

Lane J: Human B7-2-His(1ng)

Lane K: Human B7-2-His(0.5ng)

Lane L: Human B7-2-His(0.2ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17944

Recognize the protein with 10×His tag.

Anti-10×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: Human IL2Ra-His(50ng)

Lane B: Human IL6R-His(50ng)

Lane C: Human AZGP1-His(100ng)

Lane D: Human ART3-His(20ng)

Lane E: Human LILRA3-His(20ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17943

Recognize the protein with 6×His tag.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: His-Mouse TNFSF9(50ng)

Lane B: His-Human AKR1C2(200ng)

Lane C: His-Human LYPLA2(50ng)

Lane D: Human MOG-His(100ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: Human IL2Ra-His(50ng)

Lane B: Human IL6R-His(50ng)

Lane C: Human AZGP1-His(100ng)

Lane D: Human ART3-His(20ng)

Lane E: Human LILRA3-His(20ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17942

Negative binding protein without His tag.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: Human C100RF54-Fc(100ng)

Lane B: Rat B7H5-Fc(100ng)

Lane C: Rhesus C9H10orf54-Fc(100ng)

Lane D: S3-Mouse ALK6-Fc(100ng)

Lane E: Human LAG3-Fc(100ng)

Lane F: GST-Human MKI67(100ng)

Lane G: GST-Human CD247(100ng)

Lane H: MYC-GST(100ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17941

Recognize protein with His tag (internal).

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: TrxA-His-EK-Human APOE(100ng)

Lane B: TrXA-His-EK-Human BCL6(100ng)

Lane C: Trx-His-EK-Human SOCS3(100ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17940

Recognize His tag at C-terminal of the protein.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: Human LRG1-His(50ng)

Lane B: Human 4-1BB-His(50ng)

Lane C: Human SLAMF1-His(50ng)

Lane D: Human ALPL-His(100ng)

Lane E: Human IL2Ra-His(50ng)

Lane F: Human IL6R-His(50ng)

Lane G: Human AZGP1-His(100ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17939

Recognize His tag at N-terminal of the protein.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Sample:Recombiant Protein

Lane A: His-Human TPO(100ng)

Lane B: His-Mouse CLEC7A(200ng)

Lane C: His-Human LYPLA2(50ng)

Lane D: His-Human COCH(200ng)

Lane E: His-Mouse TFRC(100ng)

Lane F: His-Human HSPA1A(100ng)

Lane G: His-Human PA2G4(50ng)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17938

Recognize His tag fusion proteins which expressed by Baculovirus.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Lane A: Negative control

Lane B: His-Human LYPLA2 transfected Baculovirus Lysate(2ug)

Lane C: Human AZGP1-His transfected Baculovirus Lysate(2ug)

Lane D: Human IL2Ra-His transfected Baculovirus Lysate(2ug)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17937

Recognize His tag fusion proteins which expressed by E. coli.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Lane A: Negative control

Lane B: Human M2E1-His transfected E.coli Lysate(0.5ug)

Lane C: His-sSPA transfected E.coli Lysate(0.5ug)

Lane D: His-Human GH1 transfected E.coli Lysate(0.5ug)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Western blot (WB) 17936

Recognize His tag fusion proteins which expressed by 293 cell.

Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.

Lane A: Negative control

Lane B: His-Human LYPLA2 transfected 293 Cell Lysate(2ug)

Lane C: His-Human AKR1C2 transfected 293 Cell Lysate(2ug)

Lane D: Human LILRA3-His transfected 293 Cell Lysate(2ug)

Secondary

Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

His tag Background Information

The hexahistidine tag (6× His-tag) is the most frequently used affinity tag for protein enrichment. His-tagged proteins can be purified easily by the chelated metal ions as affinity ligands. The basis for affinity purification is known as immobilized metal affinity chromatography (IMAC). His-tag can bind best to IMAC resin in near-neutral buffer conditions (physiologic pH and ionic strength), and thus the fusion proteins can be eluted with binding buffer containing certain concentrations of imidazole. If possible, the elution is also accomplished with low pH (e.g., 0.1 M glycine-HCl, pH 2.5) or an excess of strong chelator (e.g., EDTA).
References
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Lin Z, et al. (2015) Aggregating tags for column-free protein purification. Biotechnol J 10 (12): 1877-1886.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
  • Anti-His tag Antibody (HRP)
    【Mouse】【Monoclonal】【WB,ELISA】
    105327-MM02T-H
  • Recombinant baculovirus BacCarassius-D4ORFs has potential as a live vector vaccine against CyHV-2
    Author
    Li, K;Yuan, R;Zhang, M;Zhang, T;Gu, Y;Zhou, Y;Dai, Y;Fang, P;Feng, Y;Hu, X;Cao, G;Xue, R;Chen, H;Gong, C;
    Year
    2019
    Journal
    Fish & Shellfish Immunology
    Application
    WB
    PubMed ID: 31163296FoldedExpand
  • 微小隐孢子虫 Ⅰ 型跨膜蛋白 Cgd7_4560 的亚细胞定位及其对 HCT-8 细胞的黏附特性
    Author
    邵清延, ;王科杰, ;王东强, ;张天宇, ;焦新, ;
    Year
    2019
    Journal
    吉林农业大学
    Application
    WB
    PubMed ID: 31214999FoldedExpand

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