商品介绍
CD83 Matched ELISA Antibody Pair Set,Human General Information
Product name
CD83 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human CD83
Assay range
6.25-400 pg/mL
Materials provided
1. Capture Antibody 0.5 mg/mL of mouse anti-Human CD83 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11763-MM11)2. Detection Antibody 0.2 mg/mL of mouse anti-Human CD83 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11763-MM08)3. Standard Each vial contains 13 ng of recombinant Human CD83. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
Product overview
This CD83 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CD83 . It contains Human CD83 capture antibody, Human CD83 detector antibody (HRP)and a highly purifiedHEK293-expressedrecombinant Human CD83 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD83 Matched ELISA Antibody Pair Set,Human Images
CD83 Matched ELISA Antibody Pair Set,Human: Synonyms
BL11 Matched ELISA Antibody Pair Set, Human; HB15 Matched ELISA Antibody Pair Set, Human
CD83 Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD83 is considered as a marker of mature dendritic cells as well as an adhesion receptor that binds to resting monocytes and a subset of activated CD8+ T cells. In certain conditions, CD83 tended to dimerize or even multimerize through its aberrant intermolecular disulfide bonds. The injection of CD83-Ig can significantly enhance the rate of tumor growth and inhibit the T cell growth.
Full Name
CD83 molecule
References
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
- Scholler N, et al.(2002) Cutting Edge: CD83 Regulates the Development of Cellular Immunity. The Journal of Immunology. 168 (6): 2599-602.
- Lechmann M, et al.(2002) CD83 on dendritic cells: more than just a marker for maturation. Trends in immunology. 23(6): 273-5.
- The Analysis of CD83 Expression on Human Immune Cells Identifies a Unique CD83+-Activated T Cell PopulationAuthorJu, X;Silveira, PA;Hsu, WH;Elgundi, Z;Alingcastre, R;Verma, ND;Fromm, PD;Hsu, JL;Bryant, C;Li, Z;Kupresanin, F;Lo, TH;Clarke, C;Lee, K;McGuire, H;Fazekas de St Groth, B;Larsen, SR;Gibson, J;Bradstock, KF;Clark, GJ;Hart, DN;Year2016JournalJ. Immunol.ApplicationELISAPubMed ID: 27837105FoldedExpand
- Expression of soluble CD83 in plasma from early-stage rheumatoid arthritis patients is not modified by anti-TNF-α therapyAuthorKristensen, AM;Stengaard-Pedersen, K;Hetland, ML;Hørslev-Petersen, K;Junker, P;Østergaard, M;Höllsberg, P;Deleuran, B;Hvid, M;Year2017JournalCytokineApplicationELISAPubMed ID: 28267648FoldedExpand
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