商品介绍
CD16a Protein, Human, Recombinant (ECD, F176V, His Tag): Product Information
Purity
> 97 % as determined by SDS-PAGE
Endotoxin
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Activity
1. Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 80 nM.2. Immobilized Human CD16a His (Cat: 10389-H08H1) at 2 μg/ml (100 μl/well) can bind Human IgG1 (Cat: 10702-HNAC), the EC50 of Human IgG1 is 150-450 ng/mL.
Protein Construction
A DNA sequence encoding the extracellular domain (Met1-Gln208) of human CD16a (P08637-1, with natural variant Phe 176 Val) was expressed with a C-terminal polyhistidine tag.
Accession#
P08637-1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gly 17
Molecule Mass
The secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human CD16a is approximately 44.2 KDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD16a Protein, Human, Recombinant (ECD, F176V, His Tag): Images
Immobilized Human CD16a His (Cat: 10389-H08H1) at 2 μg/ml (100 μl/well) can bind Human IgG1 (Cat: 10702-HNAC), the EC50 of Human IgG1 is 150-450 ng/mL.
CD16a Protein, Human, Recombinant (ECD, F176V, His Tag): Synonyms
CD16 Protein, Human; CD16A Protein, Human; Fc gammaRIIIa Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCGRIII Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIA Protein, Human; IGFR3 Protein, Human; IMD20 Protein, Human
CD16a Background Information
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Full Name
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Research Areas
- Fc Receptors
- Cancer Drug Targets
- ITIM/ITAM Immunoreceptors and Related Molecules
References
- David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
Human CD16a Proteins
- CD16a Protein, Human, Recombinant (176 Val, His & AVI Tag)10389-H27H1
- CD16a Protein, Human, Recombinant (ECD, F176V, His & AVI Tag), Biotinylated10389-H27H1-B
- CD16a Protein, Human, Recombinant (ECD, 176F, His & AVI Tag), Biotinylated10389-H27H-B
- CD16a Protein, Human, Recombinant (ECD, 176 Phe, His & AVI Tag)10389-H27H
- CD16a Protein, Human, Recombinant (ECD, 176 Phe, His Tag)10389-H08C
- CD16a Protein, Human, Recombinant (ECD, F176V, His Tag)10389-H08C1
- CD16a Protein, Human, Recombinant (ECD, 176F, His Tag)10389-H08H
Rat CD16a Proteins
- CD16a Protein, Rat, Recombinant (His Tag)80017-R08H
Cynomolgus CD16a Proteins
- CD16a Protein, Cynomolgus, Recombinant (His Tag), Biotinylated90013-C08H1-B
- Bispecific igg antibodies as t cell engagersAuthorABH Bakker, PF Van Loo…Year2013JournalUS 20140120096 A1FoldedExpand
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