Sino Biological/EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag/1/MG51091-CF,Sino Biological,,Sino Biological,sinobiological,+,,Array.Array蚂蚁淘厂家直采.

产品名称：Sino Biological/EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag/1/MG51091-CF

产品编号：MG51091-CF

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>聚合物>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Sino Biological

公司：SinoBiological

公司分类：

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EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: General Information

Gene

Species

Mouse

NCBI Ref Seq

NM_207655.2

RefSeq ORF Size

3633 bp

Sequence Description

Identical with the Gene Bank Ref. ID sequence except for the point mutations: 40 T>C, 1725 A>G and 2799 G>A not causing the amino acid variation.

Description

Full length Clone DNA of Mouse epidermal growth factor receptor with C terminal Flag tag.

Plasmid

Promoter

Enhanced CMV promoter

Vector

pCMV3-C-FLAG

Restriction Sites

KpnI + NotI (6kb + 3.68kb)

Tag Sequence

FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG

Sequencing Primers

T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Kanamycin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

EGFR cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: Validated Images

Mouse EGFR/HER1 Gene Expression validated Image 2265

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: Synonyms

9030024J15Rik cDNA ORF Clone, Mouse; AI552599 cDNA ORF Clone, Mouse; Erbb cDNA ORF Clone, Mouse; Errb1 cDNA ORF Clone, Mouse; Errp cDNA ORF Clone, Mouse; wa-2 cDNA ORF Clone, Mouse; wa2 cDNA ORF Clone, Mouse; Wa5 cDNA ORF Clone, Mouse

EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Cancer Immunotherapy
Immune Checkpoint
Immunotherapy
Targeted Therapy

Full Name

epidermal growth factor receptor

Research Areas

Cancer Drug Targets
Receptor Tyrosine Kinases (RTKs)

Related Pathways

EGFR Signaling Pathway
Jak-Stat Signaling Pathway

References

Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

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TFF3 is a ligand for LINGO2 that de-represses EGFR to control disease outcome during colitis and gastrointestinal nematode infection
Author
Ji, Y;Wei, Y;Park, J;Hung, L;Young, T;Herbine, K;Oniskey, T;Pastore, C;Nieves, W;Somsouk, M;Herbert, D;
Year
2018
Journal
bioRxiv
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品牌介绍

中国生物科技股份有限公司是全球最大的cDNA、重组蛋白和抗体生产商之一。在流感病毒和其他致病性病毒的研究和开发方面，华生物正在迅速领先世界。中生生物所有试剂产品均由公司生产和质量控制，质量保证优于世界其他试剂供应商。它提供了不同菌株的各种抗体、蛋白质和cDNA克隆。临床前动物试验中使用了100多种抗体，抗体产品的检测灵敏度是国际主流试剂供应商的2-10倍

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